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Lipofectamine® 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine® 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene expression studies. Lipofectamine® 2000 Transfection Reagent is our best transfection reagent available for the co-transfection of siRNA and plasmid DNA.
With Lipofectamine® 2000 Transfection Reagent, you'll get:
• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines
机理:Lipofectamine™ 2000转染试剂是一种阳离子脂质体,在体外可以转染许多种哺乳动物细胞，对各类细胞系都可提供最高的转染效率，具有高效性和操作步骤简单的优点。其转染效率以及细胞活性取决于许多实验因素,如细胞密度、脂质体和DNA的浓度、脂质体2DNA复合物的作用时间以及是否有其它介质成分如抗生素和血清。
使用Lipofectamine™ 2000试剂，您可以获得：
*在多种细胞系中具有极高地转染效率和较高水平地重组蛋白表达
*传输siRNA的优异性能
*在有血清存在的情况下可保证转染效力——转染后无需更换培养基
*保证可以用于高通量应用的试剂
*用于建立稳定细胞系的可靠试剂Lipofectamine™ 2000试剂为大多数细胞提供了最高的转染效率和活性。当存在血清或用于进行蛋白表达的细胞系（如COS-7，CHO和293）时，Lipofectamine™ 2000尤为有效，效率可超过90%。
| Cell Type: | Established Cell Lines, Primary Cells, Stem Cells, Hard-to-Transfect Cells |
|---|---|
| Sample Type (Specific): | Plasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR) |
| Transfection Technique: | Lipid-Based Transfection |
| Serum Compatible: | Yes |
| Format: | Vial(s) |
| Product Size: | 0.3 mL |
| Shipping Condition: | Approved for shipment at Room Temperature or on Wet Ice |
| High Throughput Compatibility: | High Throughput-Compatible |
